new trends in advanced fluorescence techniques

january 25-29, 2016 | concepción, chile

This course will consist of theoretical lectures, a hands-on laboratory course and computer-based training on data analysis and simulations. It will focus on the basics and advanced concepts of fluorescence spectroscopy techniques. The laboratory course is designed for students, researchers and industrial scientists who intend to extend their knowledge of fluorescence spectroscopy techniques.

Key scientists in the field will deliver theoretical lectures. The lectures will be complemented by the direct utilization of fluorescence instrumentation. Topics addressed in this course include:

  1. Basic Definitions and Principles of Fluorescence
    • Basic Spectral Properties
    • Perrin-Jablonski Diagram and Stokes' shift
    • Excitation and Emission Spectra
    • Polarization/Anisotropy
    • Fluorescence Lifetime
    • FRET: Fӧrster Resonance Energy Transfer
  2. Confocal and 2-Photon Fluorescence Microscopy
    • Instrumentation
    • Light Sources: One-photon and Multi-photon Excitation
    • Advantages and Disadvantages
  3. FCS (Fluorescence Correlation Spectroscopy)
    • Basic Principles of FCS
    • Autocorrelation and PCH Analysis
    • FCS in Solutions and in Cells
  4. RICS (Raster Image Correlation Spectroscopy)
    • Basic Concepts
    • RICS in Solutions and Cells
  5. N&B (Number and Brightness Analysis)
    • Basic Concepts
    • N&B in Solutions and Cells
  6. FLIM (Fluorescence Lifetime Microscopy)
    • The Phasor Approach
    • Applications
  7. Particle Tracking
  8. Spectral Phasor
  9. Practical Use of Instrumentation
  10. Overview of Fluorescence Probes and Labeling of Biomolecules - in vitro and in cells

Theoretical classes are open but registration is needed. The hands-on laboratory course is limited to 20 participants.