principles of fluorescence techniques

april 17-20, 2023 | urbana-champaign, il

• Basic Definitions and Principles of Fluorescence
  • Basic Spectral Properties
  • Jablonski Diagram and Stokes' shift
  • Excitation and Emission Spectra
  • Polarization/Anisotropy
  • Fluorescence Lifetime
  • FRET: Fӧrster Resonance Energy Transfer
• Instrumentation
  • Steady-State Fluorometer
  • Instrumentation for Time-Resolved Fluorescence
  • Light Sources for Time-Resolved Fluorometry (lamps, lasers, laser diodes, LEDs, synchrotron radiation)
  • Detectors (PMT, APD, MCP)
• Time-dependent Phenomena (Part I)
  • Multi-Exponential Decays
  • Time-Domain Lifetime Measurements
  • Frequency-Domain Lifetime Measurements
  • Quenching, Static, Dynamic, Transients
• Time-dependent Phenomena (Part II)
  • Anisotropy Decays
  • Energy Transfer-Distance Distributions
  • Time-Dependent Spectral Relaxation
  • Excited State Reactions
• Data Manipulation and Data Analysis
  • Spectral Manipulation
  • Least-square Analysis
  • Global Analysis
  • Phasor Plots
• Analytical Applications of Fluorescence
  • Advantages of Fluorescence in Chemical Analysis
  • Examples of Fluorescence Assays
  • Error Sources in Fluorescence Assays
  • Methods of Fluorescence Sensing
  • Lifetime-Based Sensing
  • Lifetime Sensing and Ratiometric Probes
• Confocal Fluorescence Microscopy
  • Instrumentation
  • Light Sources: One-photon and Multi-photon Excitation
  • Applications in Cells
  • Lifetime Imaging
• Fluorescence Fluctuations Spectroscopy (FFS)
  • FFS in Solutions
  • FFS in Cells
  • Single-Molecule Fluorescence
• Super-resolution Microscopy
• Practical Use of Instrumentation