Notes

Slide Show

Outline

1	Principals of Fluorescence Techniques
2	“Lifetime” of a Fluorophore
3	Radiative/Intrinsic Lifetime
4	Intensity-Decay Curve
5	Strickler-Berg Equation
6	Environment and Lifetime
7	Time Domain/Frequency Domain Methods
8	Fluorescence Lifetime
9	Deconvolution Method
10	Multiexponential Decay
11	TCSPC Method
12	Decay Data
13	Harmonic Method
14	Harmonic Method II
15	Modulations
16	Phase and Modulation
17	Fractional Contribution & Total Intensity
18	Plot
19	Multi-exponential Decay
20	Distribution and Decay Models
21	Quenching I
22	Quenching II
23	Static Quenching I
24	Static Quenching II
25	Static Quenching III
26	Static Quenching IV
27	Static Quenching V
28	Static Quenching VI
29	Fluorescence Resonance Energy Transfer
30	FRET
31	Energy Diagram
32	Energy Diagram II
33	Rate of Transfer
34	Overlap Integral
35	Orientation Factor
36	Orientation Factor II
37	Orientation Factor III
38	Orientation Factor IV
39	Energy Transfer Efficiency
40	Distance Dependence
41	FRET Experiments in vitro I
42	FRET Experiments in vitro II
43	FRET Experiments in vivo
44	Homo-transfer of Electronic Excitation Energy
45	Excitation Transfer
46	Weber’s Red-Edge Effect
47	Plot
48	Temperature Dependence
49	Effeciency of Transfer
50	Dimer/Monomer Equilibrium
51	Homotransfer
52	Homotransfer II
53	Sources