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new trends in advanced fluorescence techniques
january 25-29, 2016 | concepción, chile
Basic Definitions and Principles of Fluorescence
Basic Spectral Properties
Perrin-Jablonski Diagram and Stokes' shift
Excitation and Emission Spectra
Polarization/Anisotropy
Fluorescence Lifetime
FRET: Fӧrster Resonance Energy Transfer
Confocal and 2-Photon Fluorescence Microscopy
Instrumentation
Light Sources: One-photon and Multi-photon Excitation
Advantages and Disadvantages
FCS (Fluorescence Correlation Spectroscopy)
Basic Principles of FCS
Autocorrelation and PCH Analysis
FCS in Solutions and in Cells
RICS (Raster Image Correlation Spectroscopy)
Basic Concepts
RICS in Solutions and Cells
N&B (Number and Brightness Analysis)
Basic Concepts
N&B in Solutions and Cells
FLIM (Fluorescence Lifetime Microscopy)
The Phasor Approach
Applications
Particle Tracking
Spectral Phasor
Practical Use of Instrumentation
Overview of Fluorescence Probes and Labeling of Biomolecules - in vitro and in cells