advanced applications of fluorescence techniques

july 4-6, 2016 | avellino, italy

• Basic Fluorescence Instrumentation
  • Schematic of a Spectrofluorometer
  • Light Sources
  • Detectors
  • Monochromators
  • Filters
  • Lifetime Instrumentation
• Basic Fluorescence Principles I
  • Excitation and Emission Spectra
  • Quantum Yield
  • Polarization/Anisotropy
• Basic Fluorescence Principles II
  • Time-Resolved Fluorescence (frequency- and time-domain)
  • Anisotropy Decay
  • Quenching
  • Data Analysis (fitting algorithm and phasors)
• Basic Fluorescence Principles III
  • FRET
  • In Vitro and In Vivo Fluorescence Probes
• Microscopy Instrumentation
  • Fluorescence Microscope
  • Confocal Microscope
  • Contrast, Resolution, PSF
  • Multiphoton Excitation
• Fluorescence Applications in Biology and Medicine
• Quest for Higher Sensitivity in Fluorescence Based Detection. Developing Ultrasensitive Biomedical Assays
• Studying Cellular Functions in Real-Time and Higher Resolution
• Superresolution