advanced fluorescence microscopy techniques

december 12-14 and 17, 2011 | buenos aires, argentina

application deadline: september 30, 2011

1. Basic Definitions and Principles of Fluorescence
   • Basic Spectral Properties
   • Jablonski Diagram and Stokes' shift
   • Excitation and Emission Spectra
   • Polarization/Anisotropy
   • Fluorescence Lifetime
   • FRET: Fӧrster Resonance Energy Transfer
2. Confocal and 2-photon Fluorescence Microscopy
   • Instrumentation
   • Light Sources: One-photon and Multi-photon Excitation
   • Applications in Cells
3. FCS (Fluorescence Correlation Spectroscopy)
   • Autocorrelation and PCH
   • FCS in Solutions
   • FCS in Cells
   • RICS (Raster Image Correlation Spectroscopy)
   • N&B (Number and Brightness Analysis)
   • FLIM (Fluorescence Lifetime Microscopy) and the Phasor Approach
   • Particle Tracking
   • Practical Use of Instrumentation
   • Super Resolution Techniques